Cristofori-Armstrong, Ben
Budusan, Elena
Smith, Jennifer J.
Reynaud, Steve
Voll, Kerstin
Chassagnon, Irène R.
Durek, Thomas
Rash, Lachlan D. http://orcid.org/0000-0003-3616-0279
Funding for this research was provided by:
The University of Queensland
Article History
Received: 22 January 2024
Revised: 19 April 2024
Accepted: 12 May 2024
First Online: 17 June 2024
Declarations
:
: Animal care and procedures were carried out in strict accordance with the recommendations in the Australian code of practice for the care and use of animals for scientific purposes (8th Edition, 2013). The Anatomical Biosciences group of the Animal Ethics Committee at The University of Queensland approved surgery protocols (Approval numbers: 2018/AE000421 and 2019/AE000097). Animal surgery was performed under anaesthesia (frogs were bathed in ice-cold water containing 1.3 mg/ml of buffered MS-222, ethyl 3-aminobenzoate methane sulfonate), and all efforts were made to minimise animal suffering. Three months was the minimum time between surgeries on the same frog, and after the final surgery (maximum of six surgeries per animal) frogs were euthanised by decapitation under ice and MS-222 anaesthesia.
: Author LDR is a co-inventor on a patent (US 10,485,847) for the use of the ASIC inhibitory peptide Hi1a as a neuroprotective agent. All other authors have no relevant financial or non-financial interests to disclose.