Ren, Lipeng http://orcid.org/0000-0001-5746-3434
Charbord, Jérémie http://orcid.org/0000-0002-8411-8182
Chu, Lianhe
Kemas, Aurino M. http://orcid.org/0000-0001-9031-4748
Bertuzzi, Maria http://orcid.org/0000-0001-9444-6166
Mi, Jiarui
Xing, Chen
Lauschke, Volker M. http://orcid.org/0000-0002-1140-6204
Andersson, Olov http://orcid.org/0000-0001-6715-781X
Funding for this research was provided by:
Karolinska Institutet
Cancerfonden
H2020 European Research Council (772365)
Vetenskapsrådet
China Scholarship Council
Innovative Medicines Initiative (875510)
Karolinska Institute
Article History
Received: 7 March 2023
Accepted: 10 August 2023
First Online: 16 October 2023
Acknowledgements
: We thank D. Stainier’s lab at Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany, for sharing the <i>ins</i> mutant zebrafish; A. El Manira at Karolinska Institutet for suggestions on and making equipment available for calcium imaging; I. Valladolid Acebes, M. Köhler, N. Moruzzi and P.-O. Berggren from the Beta Cell in-vivo Imaging/Extracellular Flux Analysis core facility at Karolinska Institutet for their assistance; C. Karampelias at Karolinska Institutet for helping to set up the glucose uptake experiments in zebrafish larvae; and K.-C. Liu at Karolinska Institutet for comments on the manuscript. The graphical abstract was created with BioRender.com.
: Raw and processed RNA-seq data for this study have been deposited in the Gene Expression Omnibus under accession number GSE235398 (ExternalRef removed).
: This work was supported by grants from the European Research Council (ERC), the Swedish Research Council and Cancerfonden; by a Karolinska Institutet Doctoral grant (KID); by a grant from the China Scholarship Council (CSC); and by the Innovative Medicines Initiative 2 Joint Undertaking EUbOPEN under grant agreement No. 875510.
: VML is a co-founder and shareholder of HepaPredict AB. The authors declare that there are no other relationships or activities that might bias, or be perceived to bias, their work.
: LR performed and analysed the experiments using zebrafish and mouse islets. JC generated the <i>Tg(ins:nLuc).</i> LR, LC and JC performed in vivo mouse experiments. AMK, CX and VML performed and analysed the experiments with human hepatocytes. MB performed live calcium imaging of zebrafish larvae and analysed the experiments. JM generated <i>Tg(ins:H2BmCherry)</i>, heatmaps and PCA plots from RNA-seq data. LR, JC and OA designed the study. LR and OA wrote the manuscript with input from all authors. All authors contributed to manuscript revisions and approved the final version of the article. OA is the guarantor of the work.