Coomans de Brachène, Alexandra http://orcid.org/0000-0002-4361-5078
Alvelos, Maria Ines
Szymczak, Florian http://orcid.org/0000-0002-5431-270X
Zimath, Priscila L. http://orcid.org/0000-0003-2798-7370
Castela, Angela
Marmontel de Souza, Bianca
Roca Rivada, Arturo
Marín-Cañas, Sandra
Yi, Xiaoyan
Op de Beeck, Anne
Morgan, Noel G.
Sonntag, Sebastian
Jawurek, Sayro
Title, Alexandra C.
Yesildag, Burcak
Pattou, François
Kerr-Conte, Julie
Montanya, Eduard
Nacher, Montserrat
Marselli, Lorella
Marchetti, Piero
Richardson, Sarah J.
Eizirik, Decio L.
Funding for this research was provided by:
Leona M. and Harry B. Helmsley Charitable Trust
European Federation of Pharmaceutical Industries and Associations
Wallonie-Bruxelles International
Diabetes Fonds (Innovate2CureType1)
Instituto de Salud Carlos III, co-financed by the European Regional Development Fund (PI19/00246, PI22/00334)
INNODIA (115797)
China Scholarship Council
INNODIA HARVEST (945268)
Juvenile Diabetes Research Foundation International (3-SRA-2022-1201-S-B (2), 3-SRA-2022-1201-S-B(1))
Fonds De La Recherche Scientifique - FNRS (35248676)
HIRN-CBDS (U01 DK127786)
National Institute for Health and Care Research Exeter Biomedical Research Centre
National Institute of Diabetes and Digestive and Kidney Diseases (RO1DK126444, RO1DK133881-01)
Fondation ULB
Walloon excellence in life sciences and biotechnology (WELBIO-CR-2019C-04)
Article History
Received: 28 August 2023
Accepted: 3 January 2024
First Online: 26 February 2024
Acknowledgements
: The authors are grateful to A. M. Musuaya, L. Sauvage, Y. Cai and I. Millard of the ULB Center for Diabetes Research, Université Libre de Bruxelles, Belgium, for excellent technical support, and to J. Hopkinson, C. Flaxman, J. Hill, R. Wyatt, M. Russell and K. Murrall from EXCEED for imaging assistance.
: All data are available in the main text or the (ESM). They are available from the corresponding authors upon reasonable request. All newly generated RNA-seq data that support the findings of the present study have been deposited at GEO under accession code GSE235683.
: DLE acknowledges the support of grants from JDRF International (3-SRA-2022-1201-S-B [1] and 3-SRA-2022-1201-S-B [2]); Welbio-FNRS (Fonds National de la Recherche Scientifique) (WELBIO-CR-2019C-04), Belgium; the Dutch Diabetes Research Foundation (Innovate2CureType1), Holland; JDRF (3-SRA-2022-1201-S-B); the National Institutes of Health Human Islet Research Network Consortium on Beta Cell Death & Survival from Pancreatic β-Cell Gene Networks to Therapy (HIRN-CBDS) (grant U01 DK127786); and the National Institutes of Health NIDDK grants RO1DK126444 and RO1DK133881-01. DLE, PM, SJR and NGM acknowledge support from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreements 115797 (INNODIA) and 945268 (INNODIA HARVEST). These joint undertakings receive support from the European Union’s Horizon 2020 research and innovation programme and the European Federation of Pharmaceutical Industries and Associations (EFPIA), JDRF and The Leona M. and Harry B. Helmsley Charitable Trust. FS is supported by a Research Fellow (Aspirant) fellowship from the FNRS (Belgium). XY is supported by the Fondation ULB, Wallonie-Bruxelles International (WBI) and the China Scholarship Council. AOdB is supported by a grant (CDR) from the FNRS (35248676). EM acknowledges the support of grants PI19/00246 and PI22/00334 from Instituto de Salud Carlos III, co-financed by the European Regional Development Fund (ERCF). Parts of this study were supported by the National Institute for Health and Care Research Exeter Biomedical Research Centre. The views expressed are those of the authors and not necessarily those of the NIHR or the Department of Health and Social Care.
: SS, SJ and ACT are or were employees of InSphero AG, a company commercialising islet microtissues and related services. BY is a member of the management team of InSphero AG. DLE is a member of the Scientific Advisory Board of InSphero AG. PM is a member of the editorial board of <i>Diabetologia</i>. The other authors declare that there are no relationships or activities that might bias, or be perceived to bias, their work.
: ACdB, MIA and FS contributed to the original idea; to the design, performance and interpretation of the experiments; and to the investigation and formal analysis; and wrote, revised and edited the manuscript. PLZ performed and analysed experiments, and wrote, revised and edited the manuscript. AC, BMdS, SJR, NGM, ARR, SM-C, XY, AOdB, SS, SJ, ACT and BY performed and analysed experiments. FP, JK-C, EM, MN, LM and PM contributed with material and acquisition of data. DLE contributed to the original idea and the design, supervision and interpretation of the experiments, and wrote and revised the manuscript. All authors have read and approved the final version of the manuscript. ACdB, MIA and DLE are the guarantors of this work and, as such, have full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.