Lithovius, Väinö http://orcid.org/0000-0002-1537-5548
Lahdenpohja, Salla http://orcid.org/0000-0003-2456-5009
Ibrahim, Hazem http://orcid.org/0000-0003-0165-2660
Saarimäki-Vire, Jonna http://orcid.org/0000-0002-8865-7060
Uusitalo, Lotta
Montaser, Hossam http://orcid.org/0000-0003-0879-1807
Mikkola, Kirsi http://orcid.org/0000-0001-5074-9675
Yim, Cheng-Bin
Keller, Thomas
Rajander, Johan http://orcid.org/0000-0003-3591-0963
Balboa, Diego http://orcid.org/0000-0002-4784-5452
Barsby, Tom http://orcid.org/0000-0002-9657-1371
Solin, Olof
Nuutila, Pirjo http://orcid.org/0000-0001-9597-338X
Grönroos, Tove J. http://orcid.org/0000-0003-4468-0805
Otonkoski, Timo http://orcid.org/0000-0001-9190-2496
Funding for this research was provided by:
Svenska Kulturfonden (141484)
Sigrid Juséliuksen Säätiö
Novo Nordisk Fonden
Seventh Framework Programme (222980, 602812)
Research Council of Finland (266891, 297466, 312437, 334310)
Helsingin ja Uudenmaan Sairaanhoitopiiri
University of Helsinki
Article History
Received: 12 January 2024
Accepted: 16 April 2024
First Online: 14 June 2024
Acknowledgements
: V. Oikonen (University of Turku) is thanked for advice regarding graft uptake calculations. O. Al-Zghool (University of Turku) and Turku PET imaging centre technicians A. Honkaniemi, M. Vehmanen, M. Eisala and P. Kotitalo are thanked for assistance in PET image acquisition and processing, and processing of grafts. S. Eurola and M. Winder (University of Helsinki) are thanked for genome editing. O. Kolari (University of Eastern Finland) is thanked for assistance in graft sectioning, immunostaining and microscopy. E. Vähäkangas, V. Chandra and E. Kuuluvainen (University of Helsinki) are thanked for feedback during the project.
: All data are available on reasonable request.
: Open Access funding provided by University of Helsinki (including Helsinki University Central Hospital).The Otonkoski laboratory has received funding for this study from: the Academy of Finland (grant 297466) and as its Center of Excellence (MetaStem, grant 312437), the Sigrid Jusélius Foundation, the Novo Nordisk Foundation and the Helsinki University Hospital Funds. The Nuutila group/Turku PET Centre has received funding for this study from: the Academy of Finland CoE, European Commission FP7 programmes BetaImage (222980) and BetaCure (602812), and the Novo Nordisk Foundation. The Solin group/Turku PET Centre has received funding for this study from: the Academy of Finland (266891 and 334310) and the Swedish Cultural Foundation in Finland (151484). VL has received personal grant support from the Emil Aaltonen Foundation, Orion Research Foundation, the Finnish Medical Foundation, the Maud Kuistila Memorial Foundation, the Ida Montin Foundation and the Biomedicum Helsinki Foundation, and gratefully acknowledges their support.
: The authors declare that there are no relationships or activities that might bias, or be perceived to bias, their work.
: VL conceptualised and coordinated the study; differentiated SC-islets; performed implantations, in vivo tests and immunohistochemistry; analysed PET imaging and immunohistochemistry data; and prepared the original manuscript and figures. SL, LU, TK, C-BY and JR synthesised the radiotracers. HI performed genome editing. JS-V and HM performed implantations and in vivo tests. KM conceptualised the study and performed PET imaging. DB analysed single-cell RNA sequencing data and edited the manuscript. TB analysed data and edited the manuscript. TJG performed PET imaging and PET data analysis, conceptualised and coordinated the study, and edited the manuscript. OS supervised radiotracer synthesis and provided resources. PN conceptualised the study and provided resources. TO conceptualised and supervised the study, edited the manuscript and provided resources. All authors reviewed the article critically for intellectual content and approved the final version to be published. VL is the guarantor for this study.