Wachowski, Nicholas A.
Pippin, James A.
Boehm, Keith
Lu, Sumei
Leonard, Michelle E.
Manduchi, Elisabetta
Parlin, Ursula W.
Wabitsch, Martin
Chesi, Alessandra
Wells, Andrew D.
Grant, Struan F. A.
Pahl, Matthew C.
Funding for this research was provided by:
Children's Hospital of Philadephia (Daniel B. Burke Endowed Chair for Diabetes Researc)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD056465)
National Institute of Diabetes and Digestive and Kidney Diseases (UM1 DK126194)
Article History
Received: 28 March 2024
Accepted: 4 July 2024
First Online: 6 September 2024
Acknowledgements
: We thank G. Costecalde from Univercell-Biosolutions for providing the EndoC-βH1 cells. We acknowledge B. Johnson from the Children’s Hospital of Philadelphia for assistance with culturing EndoC-BH1 cells.
: Raw and processed next-generation sequencing data for EndoC-BH1, HepG2, SGBS_undiff and SGBS_diff cells are deposited in GEO under the Superseries accession GSE262484. Promoter-focused Capture-C data are deposited under accession GSE262496. Hi-C data are deposited under accession GSE262481. Bulk ATAC-seq data are deposited under accession GSE262479. Bulk RNA-seq data are deposited under accession GSE262480.
: This work was supported by NIH Grants UM1 DK126194, R01HD056465 and the Center for Spatial and Functional Genomics at CHOP. SFAG is supported by the Daniel B. Burke Endowed Chair for Diabetes Research. We acknowledge that the funders had no input on the experimental design of this study.
: The authors declare that there are no relationships or activities that might bias, or be perceived to bias, their work.
: This project was conceived by SFAG. MCP and SFAG directed experimental studies. MCP conducted bioinformatic analyses and analysed experimental results. NAW designed and performed targeted knockdown and glucose-stimulated insulin secretion experiments. JAP, KB, SL, MEL and UWP designed and performed next-generation sequencing experiments. EM and AC developed the bioinformatic pipeline infrastructure. MW provided SGBS cells and was involved in data acquisition. ADW provided supervision and was involved in data acquisition. SFAG and ADW acquired funding. MCP wrote the manuscript together with NAW and SFAG. All authors contributed to, read and approved the final version of the manuscript. SFAG and MCP are guarantors of this work.