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Authors
Guccio, Nunzio
Alcaino, Constanza
Miedzybrodzka, Emily L.
Santos-Hernández, Marta
Smith, Christopher A.
Davison, Adam
Bany Bakar, Rula
Kay, Richard G.
Reimann, Frank
Gribble, Fiona M.
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Funding
Funding for this research was provided by:
Wellcome Trust (100574/Z/12/Z, 220271/Z/20/Z)
Medical Research Council (MR/M009041/1, MRC_MC_UU_00014/5, MRC_MC_UU_12012/3)
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Article History
Received: 28 June 2024
Accepted: 23 August 2024
First Online: 23 October 2024
Acknowledgements
: We thank the MRL Genomics and Transcriptomics Core, the Core Biochemical Assay Laboratory (CBAL), the Flow Cytometry Core at CIMR, the CRUK Cambridge Institute Genomics Core and Addenbrooke’s Tissue Bank. Some of the data were presented as an abstract at the 58th EASD Annual Meeting of the EASD in 2022 and the 5th European Incretin Study Group meeting in 2024.
: RNA-seq data are deposited in the National Center for Biotechnology Information–Gene Expression Omnibus (NCBI GEO) repository (GSE271017). Mass spectrometry proteomics data are deposited to the ProteomeXchange Consortium via the PRIDE partner repository (PXD052659).
: This research was funded by a Wellcome joint investigator award to FR/FMG (220271/Z/20/Z) and the MRC-Metabolic Diseases Unit (MRC_MC_UU_12012/3). NG was funded by an MRC studentship. Core support was provided by the MRC (MRC_MC_UU_00014/5) and Wellcome (100574/Z/12/Z). The LC-MS/MS instrument was funded by the MRC (MR/M009041/1).
: FMG and FR received funding from AstraZeneca and Eli Lilly for non-overlapping research on other projects. They received sponsorship from AstraZeneca, Eli Lilly, Sun Pharma and Mercodia to run the 5th European Incretin Study Group conference in Cambridge (April 2024). The authors declare that there are no other relationships or activities that might bias, or be perceived to bias, their work.
: NG generated the majority of the results and wrote the first draft of the manuscript. CA and AD did the electrophysiology. ELM, MS-H and RBB provided training and support, including for organoid maintenance, CRISPR and live-cell imaging, helping with initial data collection and analysis. RGK performed and analysed LC-MS/MS. CAS performed bioinformatics analysis of RNA-seq. FR and FMG designed and oversaw the study, revised the manuscript and are guarantors of the work. All authors contributed to manuscript revision and approved the published version.
