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Authors
Xin, Miaomiao
You, Shanshan
Wu, Jingyu
Xu, Yintai
Li, Cheng
Zhu, Bojing
Shen, Jiechen
Chen, Zexuan
Dang, Liuyi
Dan, Wei
Zhang, Xinwen
Sun, Shisheng https://orcid.org/0000-0002-7242-7164
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Article History
Received: 4 July 2022
Revised: 19 September 2022
Accepted: 22 September 2022
First Online: 1 October 2022
Declarations
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: Mice-related experiments were conducted in compliance with ethical regulations and approved by the ethics committee of Northwest University, China. Human seminal plasma-related experiments were approved by the ethics committee of Xi’an Fourth Hospital and Northwest University.
: Three mice, strain C57BL/6, male, aged 3 months, were obtained from the Central Animal Breeding House of Xi’an Jiaotong University, Xi’an, Shaanxi. Seminal plasma from six healthy men were collected in Xi’an Fourth Hospital. Semen was collected through masturbation in sterile containers after 3–7 days of sexual abstinence according to the criteria of the WHO laboratory manual. Then seminal plasma was obtained by centrifugation of semen.
: Mice were anesthetized with intraperitoneal injection of chloral hydrate, and brain tissue was removed from each mouse and cut up in PBS buffer following internationally recognized guidelines.
: The authors declare no competing interests.
