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Authors
Cinek, Ondrej
Hubáčková, Klára
Litošová, Karolína
Hlináková, Lucie
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Funding
Funding for this research was provided by:
Charles University
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Article History
Received: 10 June 2025
Accepted: 21 January 2026
First Online: 4 March 2026
Declarations
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: This is a technical report on a method development, no ethics approvals or informed consents were necessary.
: The authors declare no competing interests.
: The code used to create staggered primers described in this paper is available at along with the relevant test data. The application for drawing base frequencies over the NGS reads has also been posted at . It can be run there, provided that the free tier’s machine time limit allows it.
