Ovais, Muhammad
Khalil, Ali Talha
Raza, Abida
Islam, Nazar Ul
Ayaz, Muhammad
Saravanan, Muthupandian
Ali, Muhammad
Ahmad, Irshad
Shahid, Muhammad
Shinwari, Zabta Khan
Article History
Received: 4 December 2017
Revised: 7 March 2018
Accepted: 10 March 2018
First Online: 29 March 2018
Compliance with ethical standards
:
: The in vivo biological activities were performed on BALB/c mice of either sex weighing 25–35 g. The animals were purchased from the National Institute of Health (NIH), Islamabad. These were acclimatized in a 12-h light/dark cycle at 22 ± 2 °C for 1 week prior to experiments. The animals had ad libitum access to food and water during this period. The experimental protocols on animals were approved by the Institutional Animals Use and Care Committee and were in accordance to the NIH guidelines for the care and use of laboratory animals.Healthy adult male volunteers (ages ranging from 20 to 25 years) were recruited for the study and the inclusion was based on obtaining detailed medical history and clinical examination. The aims of the study were explained to the volunteers and informed consent was obtained from the respective participants at the start of the study. The study protocols were approved by the Institutional Ethical Committee and were in accordance with the principles of the 1964 Helsinki declaration and its later amendments.
: The authors declare that they have no conflict of interest.