Silva, Jamile R.
Sales, Natiely S.
Silva, Mariângela O.
Aps, Luana R. M. M.
Moreno, Ana C. R.
Rodrigues, Elaine G.
Ferreira, Luís C. S.
Diniz, Mariana O.
Funding for this research was provided by:
Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (2014/11073-1)
Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (2016/14344-1, 2016/11397-7, 2013/15360-2, 2015/16505-0, 2016/00708-1, 2011/51218-0)
Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (560713)
Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (520931/1996-3, 308085/2015-9)
Article History
Received: 22 May 2018
Accepted: 28 December 2018
First Online: 26 February 2019
Compliance with ethical standards
:
: The authors declare that they have no conflicts of interest.
: All handling procedures were performed according to the protocol approved by the Ethics Committee on the Use of Animals (CEUA) of the Institute of Biomedical Sciences of the University of São Paulo (Brazil) on August 18, 2014 (project number 92/2014) and according to standard rules approved by the National Council for Control of Animal Experimentation (CONCEA), Brazil.
: Six–eight-week-old C57BL/6 mice were purchased from the Animal Breeding Center of the Institute of Biomedical Sciences of the University of São Paulo (Brazil) and housed in the Animal Facility at the same Institute.
: The TC-1 cell line was originally created and kindly provided by Dr. T.C. Wu, Johns Hopkins University, Baltimore, MD, USA. The cells tested negative for mycoplasma by PCR and were cultured for no more than 2 weeks after thawing. The cells showed consistent morphologies and growth rates prior to use in in vivo experiments. The cells tested positive for the expression of the HPV-16 E6 and E7 proteins by Western blotting. The TC-1 cell line is not currently available from the ATCC bank.
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