Arranz-Nicolás, Javier
Ogando, Jesús
Soutar, Denise
Arcos-Pérez, Raquel
Meraviglia-Crivelli, Daniel
Mañes, Santos
Mérida, Isabel http://orcid.org/0000-0003-2762-6241
Ávila-Flores, Antonia http://orcid.org/0000-0001-6278-2472
Funding for this research was provided by:
Ministerio de Economía, Industria y Competitividad (MINECO); Spanish Ministry of Health (Instituto de Salud Carlos III); Madrid regional government (IMMUNOTHERCAM Consortium). (MINECO (BFU2013-47640-P); RD12/0036/0059; IMMUNOTHERCAM Consortium S2010/BMD-2326.)
Ministerio de Economía, Industria y Competitividad (MINECO); Madrid regional government (IMMUNOTHERCAM Consortium). (MINECO (SAF2014-54475-R); IMMUNOTHERCAM Consortium S2010/BMD-2326))
Article History
Received: 16 August 2017
Accepted: 19 March 2018
First Online: 23 March 2018
Compliance with ethical standards
:
: The authors declare no potential conflicts of interest.
: Mice were maintained and handled in accordance with Spanish and European directives. All procedures performed with animals were conducted according the protocols approved by the CNB/CSIC Ethics Committee on Animal Experimentation (RD53/2013). PBMC were from the Blood Transfusion Center, Red Cross (Madrid, Spain), obtained with appropriate informed consent from the donors. No personal data were registered and all procedures performed with these cells were in accordance with the ethical standards of the CNB/CSIC Ethics Committee.
: C57BL/6J-DGKαSuperscript removedmice were kindly donated by Dr. Xiao-Ping Zhong (Duke University Medical Center, Durham NC). C57BL/6J-DGKζSuperscript removedmice were a gift of Dr. Gary Koretzky (University of Pennsylvania, Philadelphia PA). These mouse lines were used to generate the corresponding OT-I DGKSuperscript removedmice. The colonies were maintained in pathogen-free conditions in the CNB animal facility, following institutional guidelines.
: Human leukemic Jurkat T cells were authenticated by polymorphic short tandem repeat (STR) locus analysis (Genomics Service, Centro de Investigaciones Biomédicas-CSIC).