Funding for this research was provided by:
National Cancer Institute (CA216234, CA013148)
Breast Cancer Research Foundation of Alabama
Text and Data Mining valid from 2018-10-17
Received: 5 December 2017
Accepted: 12 October 2018
First Online: 17 October 2018
Compliance with ethical standards
: The authors declare that they have no conflict of interest.
: All procedures involving animals were performed in accordance with the guidelines of the National Research Council (United States) Committee for the Update of the Guide for the Care and Use of Laboratory Animals and were approved by the University of Alabama at Birmingham Institutional Animal Care and Use Committee (IACUC) in protocol 09854.
: BALB/c mice were purchased from Charles River Laboratories International, Inc. BALB/c.scid mice (CBySmn.CB17-PrkdcSuperscript removedIJ) were purchased from The Jackson Laboratory.
: TS/A cells were obtained at passage 22 and passaged 2 times prior to freezing archival samples. TS/A cells were authenticated by assessing MHC haplotype via flow cytometry and by detection of antigens from murine leukemia virus. Transfected and control TS/A cells were also confirmed by gene expression of MHCII pathway gene products using nanostring assay and Western blot. Both cell lines tested negative for mycoplasma (and 13 other mouse pathogens) via PCR performed by Charles River Research Animal Diagnostic Services on June 2015.