Umezu, Daisuke
Okada, Nana
Sakoda, Yukimi
Adachi, Keishi
Ojima, Toshiyasu
Yamaue, Hiroki
Eto, Masatoshi
Tamada, Koji
Funding for this research was provided by:
Grant-in-Aid for Scientific Research (16H02474)
Ono Pharmaceutical Inc.
Article History
Received: 29 August 2018
Accepted: 16 October 2018
First Online: 24 October 2018
Compliance with ethical standards
:
: Koji Tamada received research funds from Ono Pharmaceutical Inc. Other authors declare no conflict of interest.
: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted (Yamaguchi University, Ube, Japan). Animal research was approved by the Institutional Animal Care and Use Committee of Yamaguchi University (animal research approval number: 14-001).
: Male or female 6 to 12-week-old wild-type C57BL/6 mice were purchased from Japan SLC (Shizuoka, Japan). PD-L1-KO mice with a C57BL/6 background were kindly provided by Lieping Chen.
: The MC38 mouse colon carcinoma cell line was kindly provided by F. James Primus. The 3LL mouse lung carcinoma cell line and the B16F10 mouse melanoma cell line were purchased from Japanese Collection of Research Bioresources Cell Bank and American Type Culture Collection (ATCC), respectively, who had authenticated them.