Krijgsman, Daniëlle http://orcid.org/0000-0001-6101-7146
de Vries, Natasja L.
Skovbo, Anni
Andersen, Morten N.
Swets, Marloes
Bastiaannet, Esther
Vahrmeijer, Alexander L.
van de Velde, Cornelis J. H.
Heemskerk, Mirjam H. M.
Hokland, Marianne
Kuppen, Peter J. K.
Funding for this research was provided by:
Erasmus+
Leiden International Study Fund
DOO internationalization scholarship
Prof E.L. Noach award
Kræftfonden
Article History
Received: 28 September 2018
Accepted: 16 April 2019
First Online: 3 May 2019
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: All materials were obtained after approval by the Medical Ethical Committee of the LUMC (P000.193). All procedures performed in this study were in accordance with the ethical standards of the Dutch law (“WMO”, medical research involving human subjects act), the Danish law (“LBK” nr. 1083, 15<sup>th</sup> September 2017), and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
: All CRC patients and healthy donors included in this study agreed to the use of their PBMCs and data for research purposes prior to blood sampling via written informed consent. All CRC patients and healthy donors agreed to anonymous publication of the resulting data. According to the Danish Regional Ethical Committee, assay validation does not have to be approved by the committee. Therefore, written informed consent was not required from the Danish anonymous healthy donor from which the buffy coat, included alone for validating flow cytometry, was obtained.