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Article History

Received: 21 July 2018

Accepted: 11 June 2019

First Online: 26 June 2019

Compliance with ethical standards

:

: Anandaroop Mukhopadhyay is employed by Oncosec Med Inc. The authors declare that they have no other conflicts of interest.

: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in this study involving animals were in accordance with the ethical standards of Massachusetts General Hospital (MGH), where experiments were conducted in accordance with recommendations for the proper use and care of laboratory animals as MGH is an Association for Assessment and Accreditation of Laboratory Animal Care (AALAC)-certified institution, and after IACUC approval (#2014N000059).

: C57BL/6 mice were purchased from Taconic Farms (Hudson, NY, USA, C57BL/6NTac) and the Jackson Laboratory (Farmington, CT, USA, C57BL/6J, Stock Number 000664).

: The TC-1 tumor cell lines were kindly provided by Dr. T-C Wu (Johns Hopkins Hospital, Baltimore, MD, USA). The HPV-16 E6, E7, and <i>ras</i> oncogenes were used to transform primary C57BL/6 mice lung epithelial cells to generate the TC-1 tumor cell lines as previously described []. Cell line authentication was performed by the laboratory of TC Wu, who generated these cell lines, and tested negative for mycoplasma as determined by testing performed by Taconic Laboratories.