Document is current

Article History

Received: 10 January 2019

Accepted: 16 June 2019

First Online: 28 June 2019

Compliance with ethical standards

:

: The authors declare that they have no conflict of interest.

: Experiments were approved by the institutional animal care and use committee and the Institutional Review Board at the Weizmann Institute of Science. Protocol number 40351217-2. We followed the guidelines for animal use in research in Israel and for the use of specimens of human origin.

: All patients signed a form consenting to the use of diagnostic samples taken from them for anonymous, non-commercial research purposes.

: C57BL/6 mice were purchased from Harlan laboratories Ltd. All other mouse strains were purchased from the Jackson Laboratories and bred locally.

: Cells from the B16a melanoma line [] were kindly provided by Prof. Bonnie Sloane at Wayne State University, Detroit, MI. Cells were periodically tested for the presence of mycoplasma and found clean. All lines were used within ten passages. Expression of OVA and fluorescent proteins after cell passages was confirmed by flow cytometry. All tumor lines grew rapidly in untreated immunocompetent syngeneic C57BL/6 mice and formed pigmented tumors typical of melanoma.