Funding for this research was provided by:
National Natural Science Foundation of China (81272321, 81771687, 81571532, 81601429)
Article History
Received: 2 October 2018
Accepted: 26 June 2019
First Online: 5 July 2019
Compliance with ethical standards
:
: The authors declare that they have no potential conflicts of interest.
: All mouse experiments were conducted in accordance with the “Guide for the Care and Use of Laboratory Animals” (US Department of Health and Human Services, National Institute of Health Publication no 93–23, revised 1985) and were approved by the Institutional Animal Care and Use Committee, Tsinghua University, Beijing, China. The animal research approval number is 15-ZMH1.
: Both wild-type (C57BL/6 mice) and transgenic mouse strains including mT/mG mice (B6.129(Cg)-<i>Gt(ROSA)26Sor</i><sup><i>tm4(ACTB</i>-<i>tdTomato,</i>-<i>EGFP)Luo</i></sup>/J mice), B6.GFP mice (C57BL/6-Tg(ACTB-EGFP)1Osb/J mice), OT-I mice (C57BL/6-Tg(TcraTcrb)1100Mjb/J mice), and Simian virus 40 (SV40)-specific TCR transgenic mice (B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J mice) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA) and bred in specific pathogen-free conditions in the Laboratory Animal Research Center, Tsinghua University (Beijing, China).
: Murine Yac-1, B16-F10, and EL4 cells were obtained from ATCC (Manassas, VA, USA) and the STR profiles (Supplementary Fig. InternalRef removed) of these murine cell lines, including 18-3, 9-2, 6-7, 5-5, X-1, 15-3, 12-1, 6-4, CSF1PO, vWA, 4-2 and Jarid1 loci, were detected in Beijing Microread Genetics Co., Ltd (Beijing, China). Transfection of EL4 and B16-F10 cells, for OVA<sub>257–264</sub> peptide-expressing (EL4-OVA8) cells and GFP-expressing cells (B16-GFP and EL4-OVA8-GFP), was performed in Prof. Ying Wan’s laboratory. Expressions of transfected GFP/OVA sequences were validated by flow cytometry (Supplementary Figure 3).