Makowska, Anna
Franzen, Sabrina
Braunschweig, Till
Denecke, Bernd
Shen, Lian
Baloche, Valentin
Busson, Pierre
Kontny, Udo http://orcid.org/0000-0002-3072-6772
Funding for this research was provided by:
Internal Funding, Faculty of Medicine
Article History
Received: 2 February 2019
Accepted: 5 July 2019
First Online: 16 July 2019
Compliance with ethical standards
:
: The authors declare that they have no conflicts of interest.
: The final protocol was approved by the ethics committee of the Rhenish-Westphalian Technical University, Aachen, Germany [EK 005/18]. The study was conducted in accordance with the Declaration of Helsinki (2013 revision).
: Procedures for mouse handling and xenografts were reviewed and approved by the Ethics Committee for animal experimentation n°26 (Gustave Roussy, Villejuif, France), in accordance with the European directive 2010/63/EU and the decrees of the French ministry of Agriculture R. 214-87 to R. 214-126. The approval was given in November 26, 2015, under the Number Apafis #1605—2015090216498538-v2.
: Written informed consent was obtained from all individual participants included in the study. Patients were treated at the Uniklinik RWTH Aachen. Informed consent including use of biological specimen (tumor, peripheral blood, urine) and data acquisition and processing was obtained from patients prior to the initiation of the study. Informed consent including the use of peripheral blood and anonymous processing of data was obtained from healthy volunteers who were part of the laboratory staff.
: Swiss nude mice were bred in the animal facility at Gustave Roussy.
: C666-1 was a gift from Prof. Fei–Fei Liu, University of Toronto, Canada [CitationRef removed] and the SV40T-antigen immortalized nasopharyngeal epithelial cell line NP69 [CitationRef removed] was obtained from Prof. George Tsao (The Chinese University of Hong Kong, Hong Kong, China). Cell authentication was done using short tandem repeated profiles as described previously [CitationRef removed], and cell lines were tested at regular intervals by PCR to rule out mycoplasma contamination. Authentication of C17 cell was done by checking of HLA class I alleles by PCR (A02.01/A26.01–B44.02/B51.01).