Castiello, Luciano
Zevini, Alessandra
Vulpis, Elisabetta
Muscolini, Michela
Ferrari, Matteo
Palermo, Enrico
Peruzzi, Giovanna
Krapp, Christian
Jakobsen, Martin
Olagnier, David
Zingoni, Alessandra
Santoni, Angela
Hiscott, John http://orcid.org/0000-0003-2076-6321
Funding for this research was provided by:
National Institutes of Health (7R21CA192185)
Associazione Italiana per la Ricerca sul Cancro (IG16901)
National Institutes of Health (1R561AI108861-01A1)
Article History
Received: 7 December 2018
Accepted: 15 August 2019
First Online: 28 August 2019
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: PBMC were freshly isolated from peripheral blood samples of anonymous volunteer healthy donors at the Transfusion Center of Sapienza University of Rome. Written informed consent was obtained from all blood donors to the use of their blood for scientific purposes.
: All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of the Sapienza University of Rome (Rif.3373/250914) and with the 1964 Helsinki declaration. Institutional Review Board approval was not required for this kind of study.
: Primary Mel1007 and metastatic melanoma Mel120 cells were a kind gift of Dr. G. Parmiani (Milan, Italy), A549, HCT116 and PC3 were all from ATCC. All the experiments were performed with cells at low passage numbers (≤ 10). Cell identity was monitored based on morphology and growth rate. Mycoplasma was routinely tested.