Lapenta, Caterina http://orcid.org/0000-0002-2771-6813
Donati, Simona http://orcid.org/0000-0001-5214-9913
Spadaro, Francesca http://orcid.org/0000-0002-6610-350X
Lattanzi, Laura http://orcid.org/0000-0001-6998-4827
Urbani, Francesca http://orcid.org/0000-0002-5522-2706
Macchia, Iole http://orcid.org/0000-0002-3870-8202
Sestili, Paola http://orcid.org/0000-0002-7624-1025
Spada, Massimo
Cox, Maria Christina http://orcid.org/0000-0002-8597-8459
Belardelli, Filippo http://orcid.org/0000-0002-3082-3169
Santini, Stefano M. http://orcid.org/0000-0002-7580-5589
Funding for this research was provided by:
Celgene (ITA-017)
Associazione Italiana per la Ricerca sul Cancro (IG16891)
Article History
Received: 15 February 2019
Accepted: 5 October 2019
First Online: 16 October 2019
Compliance with ethical standards
:
: Stefano M. Santini received research funding from Celgene. All other authors declare that they have no conflict of interest. Celgene had no role in study design, data collection, data interpretation, writing of the manuscript.
: All experiments utilizing PBMC from healthy donors were conducted in accordance with the ethical standards of the Ethics Committee of Istituto Superiore di Sanità and the Declaration of Helsinki. Institutional Review Board approval was not required for this kind of study. Buffy coat supply for our studies was approved by Azienda Ospedaliera Policlinico Umberto I on 24/02/2014 (aut.6802). All experiments utilizing blood samples from FL patients were conducted in accordance with the declaration of Helsinki. Study and procedures were approved by the Ethics Committee of Azienda Ospedaliera Sant’Andrea (aut.169/2011). All experiments on mice were executed in compliance with the Istituto Superiore di Sanità Service for Animal Welfare guidelines and after approval from the Italian Ministry of Health (aut. 296/2015-PR). Mice were housed according to Legislative Decree 26/2014 guideline.
: PBMC were freshly isolated from peripheral blood samples of anonymous volunteer healthy donors at the Transfusion Center of of Policlinico Umberto I—University “La Sapienza”, Rome. Written informed consent was obtained from all blood donors to the use of their blood for research and scientific purposes. Blood samples from FL patients were anonymously provided by the Hematology Unit at the Azienda Ospedaliera Sant’Andrea Rome, Italy. Written informed consent was obtained from FL patients for the use of blood and lymph node specimens in IFN-DC-based vaccine researches.
: NOD/SCID (NOD.CB17-Prkdcscid/NCrHsd) female mice were purchased from Envigo (Italy), used at 3–4 weeks of age.
: Karpas-422 FL cell line was purchased from the cell bank Interlab Cell Line Collection (ICLC). Human K562 erythroleukemic cell line was purchased from the European Collection of Authenticated Cell Culture (ECACC). Both cell lines were authenticated by the suppliers by short tandem repeat (STR) profiling. The cell lines were initially grown and cryopreserved into multiple aliquots. All the experiments were performed with cells at low passage numbers (≤ 10).