Zhou, Linlin
Ruan, Mei
Liu, Ying
Zhu, Yanyang
Fu, Deqiang
Wu, Kunlin
Zhang, Qiuyu http://orcid.org/0000-0003-4081-7480
Funding for this research was provided by:
the Natural Science Foundation of Fujian Province (2017J01525)
the Key Research Program of Fujian Provincial Heath and Education Joint Committee (WKJ2016-2-31)
Article History
Received: 23 January 2019
Accepted: 9 December 2019
First Online: 17 December 2019
Compliance with ethical standards
:
: The authors declare that they have no conflicts of interest.
: All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. All samples were obtained in accordance with the institutional policies. All protocols were reviewed and approved by the Ethical Committee of Fujian Medical University (2017-NO. 27). All procedures performed in studies involving animals were approved by the Fujian Medical University Institutional Animal Care and Use Committee (IACUC) in accordance with the ethical standards. Animal research approval number: 2017-031. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
: All patients included in this study agreed to the use of their tumor tissue samples and data for research purposes prior to surgery via written informed consent.
: Female C57BL/6 mice (on the H-2Kb background), NOD-scid IL-2Rgnull (NSG) mice and T-cell receptor transgenic mice specific for H-2Kb OVA257-264 (OT-I) at 6–8 weeks of age were purchased from Model Animal Research Center of Nanjing University. All mice were maintained in pathogen-free facilities.
: E0771 and GL261 were a generous gift from Professor Lieping Chen (Yale University, CT, USA). The phenotypes of these cell lines were verified by a Short Tandem Repeat analysis. MCF-7, SKBR-3, MDA-MB-468, EG7 and 4T1 were purchased from the American Type Culture Collection (Mabassas, USA), and these cell lines were authenticated there. Expression of B7H4 on EG7-B7H4 and GL261-B7H4 were verified by flow cytometry, as shown in Fig. InternalRef removed. These cell lines were tested mycoplasma free. Phenotypes from cell morphology and growth rate were monitored regularly and did not change over time.