Yahiro, Kenichiro
Matsumoto, Yoshihiro
Yamada, Hisakata
Endo, Makoto
Setsu, Nokitaka
Fujiwara, Toshifumi
Nakagawa, Makoto
Kimura, Atsushi
Shimada, Eijiro
Okada, Seiji
Oda, Yoshinao
Nakashima, Yasuharu
Funding for this research was provided by:
Japan society for the Promotion of Science (18K16627)
Japan Society for the Promotion of Science (19K16802, 18K09067)
Japan Orthopaedics and Traumatology Foundation (332)
Fukuoka Foundation for Sound Health
Article History
Received: 13 August 2019
Accepted: 28 January 2020
First Online: 11 February 2020
Compliance with ethical standards
:
: The authors declare no conflict of interest.
: The study was conducted in accordance with the Declaration of Helsinki and approved by the Institutional Ethical Review Board of the Kyushu University (#27–420, 2016/3/31). The animal study was approved by the Institutional Ethical Review Board of the Kyushu University (#A28-160–0, 2017–3-31). Experiments involving animals were performed in compliance with the guidelines established by the Animal Care and Use Committee and this study was approved by the Institutional Review Board at Kyushu University.
: Prior to the use of patient data and specimens in the study, written informed consent was obtained from all patients. They all agreed to use their clinical data and bio materials for this research and publication. Written informed consent was obtained also from the parents when the patient was a minor.
: C3H/HeN and C3H/HeJ mice were purchased from Kyudo (Saga, Japan).
: LM8 mouse osteosarcoma cells were purchased from RIKEN BioResource Research Center (Tsukuba, Japan). Cell line authentication of LM8 was performed by RIKEN.