Rodriguez-Barbosa, Jose-Ignacio http://orcid.org/0000-0001-7427-5654
Azuma, Miyuki http://orcid.org/0000-0002-3529-4585
Zelinskyy, Gennadiy http://orcid.org/0000-0001-6338-2382
Perez-Simon, Jose-Antonio http://orcid.org/0000-0003-3616-6101
del Rio, Maria-Luisa http://orcid.org/0000-0003-3710-2847
Funding for this research was provided by:
Fondo de investigaciones sanitarias (1300029)
CIBERONC (CB16/12/00480)
Article History
Received: 10 July 2019
Accepted: 14 February 2020
First Online: 22 February 2020
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: The Animal Welfare Committee of the University of Alcala de Henares (Madrid) in accordance with the European Guidelines for Animal Care and Use of Laboratory Animals approved all experiments with rodents (authorization # OH-UAH-2016/015).
: Eight- to 12-week-old female C57BL-6 J (B6, from Janvier Labs) and C57BL/6-Tg (TcraTcrb)1100Mjb/J (also known as OT-I mice) were used in this work. OT-I transgenic mice exhibit a rearranged TCR that recognizes OVA residues 257–264, SIINFEKL peptide in the context of H-2<sup>b</sup> [CitationRef removed]. These mice were kindly provided by Dr. David Sancho (CNIC, National Center for Cardiovascular Disease, Madrid). B6-background PD-L1<sup>−/−</sup> (B7-H1-KO) mice were originally generated by Lieping Chen [CitationRef removed].
: The EL-4 cell line is a chemically induced lymphoma cell line from C57BL/6 mice. E.G7 is a transplantable cell line derived from EL-4 thymoma cells that were transfected with a plasmid carrying a cytoplasmic version of chicken ovalbumin (OVA). Both cell lines were kindly provided by Prof. Dr. Ignacio Melero (CIMA, Navarra, Spain), who obtained them from ATCC. No cell line authentication was necessary.