Jevtovic, Andra
Pantic, Jelena
Jovanovic, Ivan
Milovanovic, Marija
Stanojevic, Ivan
Vojvodic, Danilo
Arsenijevic, Nebojsa
Lukic, Miodrag L.
Radosavljevic, Gordana D.
Article History
Received: 27 December 2018
Accepted: 15 February 2020
First Online: 13 April 2020
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: The murine melanoma B16-F1 and B16-F10 cell lines, purchased from American Type Culture Collection (ATCC, Manassas, USA), were routinely cultured. Cell culture was performed under standardized protocols to ensure that phenotypically similar cells are implanted during each experiment. Viability of cells was determined by trypan blue exclusion and only cell suspensions with > 95% viable cells were used.
: All animal care procedures and experimental protocols were performed in accordance with institutional and the official guidelines of EU Directive 2010/63/EU, at the Faculty of Medical Sciences, University of Kragujevac, Serbia. The experiments were approved by the Animal Ethics Board of the Faculty of Medical Sciences, University of Kragujevac, Serbia (01-2588, 17/03/2014). Consented melanoma patients were recruited from the Clinics of Dermatovenerology and Plastic Surgery, Military Medical Academy, Belgrade, Serbia, while healthy donors (with no prior history of cancer) were recruited on periodical systematic examinations. The study was approved by the local Research Ethics Committee, Military Medical Academy (11-03/2014).
: C57BL/6 mice were bred in animal breeding facilities of the Faculty of Medical Sciences, University of Kragujevac, Serbia.
: Written informed consent was obtained prior to sample collection from all individual participants included in the study in accordance with the Declaration of Helsinki.