Jeske, Sandra S.
Brand, Matthias
Ziebart, Andreas
Laban, Simon
Doescher, Johannes
Greve, Jens
Jackson, Edwin K.
Hoffmann, Thomas K.
Brunner, Cornelia
Schuler, Patrick J.
Funding for this research was provided by:
Deutsche Forschungsgemeinschaft (SCHU 2536/3)
Article History
Received: 30 July 2019
Accepted: 24 February 2020
First Online: 7 March 2020
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.<b>Ethical approval and ethical standards.</b>Human studies were approved by the ethics committee of the University Ulm, Helmholtzstr. 20, 89081 Ulm, Germany (#255/14). The Helsinki Declaration was followed. Mice were kept in groups of 4–5 mice in individually ventilated cages with nesting material under specific pathogen-free conditions in the animal facility at the University of Ulm. All animal experiments were performed in accordance with institutional and governmental regulations in compliance with European rules (2010/63/EU) and were approved by the regional animal ethics committee Regierungspräsidium (Tübingen, Germany), Konrad-Adenauer-Str. 20, 72072 Tübingen, Germany (TVA1270).
: Informed consent was obtained in written form from all human donors including the healthy control group before sample collection. Donors consented to the use of their specimens and data for research and for publication.
: Animals were obtained from Janvier Labs; CS 4105 Le Genest St Isle; 53941 St Berthevin Cedex, France.
: The murine SCC VII cell line, which derived from a spontaneously arising squamous cell carcinoma of C3H mice, was first described by Fu et al. [CitationRef removed], further characterized [CitationRef removed] and kindly provided by Sven Brandau [CitationRef removed] (University of Essen, Germany). The SCC VII cell line was authenticated in a bioassay, in which 1 × 10<sup>5</sup> SCC VII cells were injected subcutaneously (back/flank) of fully immunocompetent C3H/HeN mice and tumor growth was observed within 21 days post-inoculation with a size of maximal 1 cm<sup>3</sup> as described [CitationRef removed, CitationRef removed, CitationRef removed].