Diaz-Lucena, Daniela
Kruse, Niels
Thüne, Katrin
Schmitz, Matthias
Villar-Piqué, Anna
da Cunha, Jose Eriton Gomes
Hermann, Peter
López-Pérez, Óscar
Andrés-Benito, Pol
Ladogana, Anna
Calero, Miguel
Vidal, Enric
Riggert, Joachim
Pineau, Hailey
Sim, Valerie
Zetterberg, Henrik
Blennow, Kaj
del Río, Jose Antonio
Marín-Moreno, Alba
Espinosa, Juan Carlos
Torres, Juan María
Sánchez-Valle, Raquel
Mollenhauer, Brit
Ferrer, Isidre http://orcid.org/0000-0001-9888-8754
Zerr, Inga
Llorens, Franc
Funding for this research was provided by:
Instituto de Salud Carlos III (CP16/00041 and PI19/00144)
Fundació la Marató de TV3 (201821)
Poctefa (148/16)
Polarforskningssekretariatet (2017-00915)
Article History
Received: 23 February 2021
Revised: 15 March 2021
Accepted: 15 March 2021
First Online: 21 April 2021
Declarations
:
: HZ has served on scientific advisory boards for Denali, Roche Diagnostics, Wave, Samumed, Siemens Healthineers, Pinteon Therapeutics, and CogRx, has given lectures in symposia sponsored by Fujirebio, Alzecure, and Biogen, and is a co-founder of Brain Biomarker Solutions in Gothenburg AB (BBS), which is a part of the GU Ventures Incubator Programme. KB has served as a consultant, on advisory boards, and on data monitoring committees for Abcam, Axon, Biogen, Julius Clinical, Lilly, MagQu, Novartis, Roche Diagnostics, and Siemens Healthineers, and is a co-founder of Brain Biomarker Solutions in Gothenburg AB (BBS), which is a part of the GU Ventures Incubator Programme.
: Brain tissue was obtained from the Institute of Neuropathology HUB-ICO-IDIBELL Biobank and the Hospital Clinic de Barcelona-IDIBAPS Neurological Tissue Bank following the Spanish legislation guidelines on this matter (Real Decreto de Biobancos 1716/2011) and approval of the local ethics committees. CSF samples were collected at the following centres: (1) Clinical Dementia Centre and the National Reference Centre for CJD Surveillance at the University Medical Centre of Göttingen (Germany); (2) Alzheimer's Disease and Other Cognitive Disorders Unit, Hospital Clínic de Barcelona, Barcelona (Spain); (3) Multiple Sclerosis Unit Neurology Service, Bellvitge University Hospital, Hospitalet de Llobregat, Spain; (4) National Centre of Microbiology-Instituto de Salud Carlos III, Madrid, Spain; and (5) Istituto Superiore di Sanità, Rome, Italy. Blood was collected at the Department of Transfusion Medicine (healthy controls) and at the Department of Neurology (sCJD cases) of the University Medical Centre Göttingen (Germany) under standardized pre-analytical conditions.
: We conducted animal experiments in accordance with the Code for Methods and Welfare Considerations in Behavioural Research with Animals (Directive 2010/63/EU) and made every effort to minimize suffering. Experiments developed in the Centro de Investigación en Sanidad Animal–Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Madrid, Spain) were evaluated by the Committee on the Ethics of Animal Experiments of the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria and approved by the General Directorate of the Madrid Community Government (permit no. PROEX 094/18).