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Article History

Received: 23 February 2018

Accepted: 27 June 2018

First Online: 5 July 2018

Compliance with ethical standards

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: The author declares that he has no conflict of interest.

: All applicable international, national, and/or institutional guidelines for the care and use of embryonated chicken eggs were followed. All experiments were carried out in compliance with the Iranian Veterinary Organization ethic rules. According to the law, no specific authorizations were required for work performed in avian embryos before the time of hatching.The experiment was terminated on developmental day 18, 3 days before hatching, by chilling the eggs on ice for 20 min.