Suárez-Oubiña, Cristian
Mangone, Annarosa
Giannossa, Lorena C.
Nuñez-González, Laura
Herbello-Hermelo, Paloma
Bermejo-Barrera, Pilar
Moreda-Piñeiro, Antonio http://orcid.org/0000-0002-3512-6434
Funding for this research was provided by:
Universidade de Santiago de Compostela
Article History
Received: 30 March 2023
Accepted: 1 July 2023
First Online: 18 July 2023
Declarations
:
: The activities performed in the current research (fasting and sacrifice) are included in the article 1.5f of the Council Directive 2010/63/EU regarding the protection of animals used for experimental purposes, as they are considered practices not likely to cause pain, suffering, distress, or lasting harm equivalent to, or higher than, that caused by the introduction of a needle. Therefore, there was no need to have a specific approval by the competent Spanish authority to complete these experiments. Nevertheless, fish were sacrificed by personnel qualified in animal experimentation, in accordance Spanish Ministerial Order ECC/566/2015.
: The authors declare no competing interests.