Qin, Qinbo
Wang, Chongqing
Zhou, Yuwei
Qin, Huan
Zhao, Chun
Yang, Li
Yu, Tingting
Liu, Shaojun
Article History
Received: 7 November 2019
Accepted: 16 March 2020
First Online: 5 April 2020
Compliance with Ethical Standards
: Fish treatments were carried out according to the recommendations in the Guidelines for the Care and Use of Laboratory Animals of the National Advisory Committee for Laboratory Animal Research in China and approved by the Animal Care Committee of Hunan Normal University (Permit Number: 4237). Individual GRCC, RCC (♀), and BSB (♂) were randomly selected. All samples were cultured in open pools (0.067 ha) with suitable pH (7.0–8.5), water temperature (22–24 °C), dissolved oxygen content (5.0–8.0 mg/l), and adequate forage at the State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, China. All fish were anesthetized with 100 mg/l MS-222 (Sigma-Aldrich, St. Louis, MO, USA) before blood collection.
: The authors declare that they have no conflict of interest.