Funding for this research was provided by:
China’s Agricultural Research System (CARS-45-03)
Article History
Received: 27 May 2020
Accepted: 25 August 2020
First Online: 10 September 2020
Compliance with Ethical Standards
: In this study, all experiments with the fish were conducted in accordance with the guidelines on the care and use of animals for scientific purposes set up by the Institutional Animal Care and Use Committee (IACUS) of Shanghai Ocean University, Shanghai, China. The IACUS approved this study within the project “Breeding of Black Carp” (approval number SHOU-16-014). The dissection experiments were performed with 3-aminobenzoic acid ethyl ester methanesulfonate (MS-222; Sigma, USA) anesthesia to minimize the suffering of the fish.