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Authors
Ito, Gen https://orcid.org/0000-0002-9781-7206
Kuramoto, Mao
Kondo, Akihiro
Nakamura, Masafumi
Ishizaki, Daisuke
Taguchi, Takashi
Kawase, Seigo
Asami, Maki
Goto, Yuko
Yamanaka, Hiroki
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Funding
Funding for this research was provided by:
Environmental Restoration and Conservation Agency (JPMEERF20204004)
Japan Society for the Promotion of Science (22K14908)
Japan Society for the Promotion of Science (22K19346)
Japan Society for the Promotion of Science (23K05941)
Joint Research Center for Science and Technology, Ryukoku University
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Article History
Received: 25 March 2025
Revised: 30 April 2026
Accepted: 6 May 2026
First Online: 3 June 2026
Declarations
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: AK and HY, co-inventors of benzalkonium chloride usage as a preservative for eDNA samples used in this study, are affiliated with Hiyoshi Corporation and Ryukoku University, respectively, the patent owners in Japan.
: In Japan, ethical regulations regarding laboratory animals do not cover fish. However, we handled the collected individuals with ethical consideration in accordance with the Ichthyological Society of Japan's ''Guidelines for the use of fishes in research''. In addition, the collected individuals were immediately released into the field after counting the number of individuals.
