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Article History

Received: 25 May 2022

Accepted: 20 September 2022

First Online: 1 October 2022

Declarations

:

: The authors have no relevant financial or non-financial interests to disclose.

: All procedures described in this study abide with ARRIVE guidelines and are in accordance with the Brazilian Directive for the Care and Use of Animals for Scientific Purposes and Teaching and with the European Communities Council Directive of 24 November 1986 (86/609/EEC). Ethical approval was obtained via the Ethics Committee for Animal Experimentation of the State University of Londrina (process number: 15688.2017.30, official letter 112/2017). All the authors mentioned in the manuscript have agreed for authorship, read, and approved the final version of the manuscript, and given consent for submission and subsequent publication of the manuscript.

: The mice from the sample were kept under controlled conditions to guarantee their welfare: 12 h light/dark cycles, temperature set at 22 ± 2 °C and unlimited access to water and food. Besides, the animals were allocated in a proportion of two females for each male mice, in 30 cm × 20 cm × 13 cm polypropylene cages lined with wood shavings. Only two people conducted the treatments and euthanasia out of the research team, as quickly and as comfortably as possible.