Bech, N. http://orcid.org/0000-0003-3625-3686
Nivelle, D.
Caron, S.
Ballouard, J. M.
Arnal, V.
Arsovski, D.
Golubović, A.
Bonnet, X.
Montgelard, C.
Article History
Received: 16 June 2021
Revised: 5 May 2022
Accepted: 6 May 2022
First Online: 19 May 2022
Declarations
:
: We sacrificed no animals. To limit harm to tortoises, we collected small volumes (< 200 µl) of blood by a puncture of the dorsal supra-carapacial cervical plexus using 1 ml syringes and small needles (27 G and 30 G; Bonnet et al.CitationRef removed). As recommended in Beaupre et al. (CitationRef removed), we minimized handling time by not anesthetising tortoises, and cleaned the puncture wound with an antiseptic solution before releasing the tortoise.