Buono, Vincenzo
Bissattini, Alessandra Maria
Mezzasalma, Marcello
Guarino, Fabio Maria
Vignoli, Leonardo
Article History
Received: 7 December 2024
Revised: 15 April 2025
Accepted: 26 April 2025
First Online: 8 May 2025
Declarations
:
: This study was conducted according to the ethical scientific guidelines described by the American Society of Ichthyologists and Herpetologists (ASIH), The Herpetological League (HL) and the Society for the Study of Amphibians and Reptiles (SSAR).Experimental protocols were approved by Italian Ministry of the Environment that gave LV the authorization [DPN2009-0011282] to manipulate amphibians and collect tissue samples. Tissue samples were obtained with no anesthetic and by careful handling of animals. After this procedure, individuals were temporarily housed in tanks filled with water for approximately 30 min to verify their return to normal activity and released at the same point of capture. No individuals died or were otherwise harmed during samplings.All methods are reported in accordance with ARRIVE guidelines.
: The authors declare no competing interests.