Gkenas, Christos https://orcid.org/0000-0002-9863-2289
Kodde, Alexa
Ribeiro, Filipe https://orcid.org/0000-0003-3531-5072
Magalhães, Maria Filomena https://orcid.org/0000-0001-7308-2279
Funding for this research was provided by:
Fundação para a sciencia e a tecnologia (SFRH/BPD/ 84859/2012)
Fundacao para a ciencia e a tecnologia (SFRH/BPD/46761/2008, PTDC/AAG-MAA/0350/2014)
Fundação para a ciencia e a tecnologia (UID/BIA/00329/2013)
Article History
Received: 24 February 2021
Accepted: 21 July 2021
First Online: 29 July 2021
Declaration
:
: All the authors declare no conflict of interests nor competing interests.
: Fish capture, handling, housing, acclimation, feeding, experimentation, final disposition and euthanasia procedures conformed to Portuguese legislation and international guidelines on animal treatment and welfare in scientific research( htpps://doi.org/10.1016/j.anbehav.2017.10.001). All efforts were made to minimize accidental deaths and stress on fish throughout the study. At the end of experiments, pumpkinseed and the cichlid were euthanized using MS222 and chub were acclimated to ambient temperature and released at the site of collection. All fish released were in good condition and showed no injuries or contamination signs. The study was carried out under the permits of Instituto da Conservação da Natureza e das Florestas (ICNF, IP; 402/2016/CAPT and 219/2017/CAPT).
: All the authors consent to participation.
: All the authors consent to publication.