Shahbazi, Sepideh
Haghighipour, Nooshin
Soleymani, Sepehr
Nadji, Seyed Alireza
Bolhassani, Azam
Funding for this research was provided by:
Shahid Beheshti University of Medical Sciences, and Pasteur Institute of Iran
Article History
Received: 26 January 2018
Accepted: 3 April 2018
First Online: 9 April 2018
Compliance with ethical standards
:
: The authors declare that they have no competing interests.
: Physiochemical characterization and stability analysis of the +36GFP/DNA nanoparticles: A) Representative gel retardation assay of +36 GFP complexed with pcDNA-E7 at different N/P ratios (GFP: E7DNA); Lane 1: naked plasmid DNA as a control (pcDNA-E7), Lane 2: N/P = 1:1, Lane 3: N/P = 2:1, Lane 4: N/P = 5:1, Lane 5: N/P = 10:1, and Lane 6: N/P = 20:1. The DNA complexed with GFP that was not able to migrate into the gels was observed at an N/P ratio of 5:1; B) Stability analysis of GFP-based nanoparticles against DNase I; Lane 1: naked plasmid DNA with DNase, Lane 2: naked plasmid DNA without DNase, and Lane 3: N/P = 10:1.