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Authors
Shang, Jixiang
Zhang, Yanchao
Xu, Zongjun
Zhang, Shouqing
Sun, Zhongtao
Zheng, Minggang https://orcid.org/0000-0002-2538-5352
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Funding
Funding for this research was provided by:
National Key Research and Development Program of China (2018YFC0310701)
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Article History
Received: 10 March 2024
Revised: 3 October 2024
Accepted: 10 November 2024
First Online: 22 July 2025
Declarations
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: The authors declare no conflict of interest.
: Additional supporting information may be found online in the Supporting Information section at the end of the article.Appendix S1. Plasmid information of the strains used in this study. Appendix S2. Primer information required for constructing BaRecJ expression vector.Appendix S3. Background of acetic acid and ethanol tolerance in haploid and diploid yeast. Inoculate pre evolved brewing yeast into a culture medium containing acetic acid or ethanol and detect its growth. Each processing has three replicates.Appendix S4. Mutation information in the coding regions of mutant Ia-M2 and Ia-08.
