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Authors
Voleníková, Anna https://orcid.org/0000-0002-9504-8044
Lukšíková, Karolína
Mora, Pablo https://orcid.org/0000-0001-7967-3379
Pavlica, Tomáš
Altmanová, Marie https://orcid.org/0000-0001-7193-8918
Štundlová, Jana https://orcid.org/0000-0001-7890-1608
Pelikánová, Šárka
Simanovsky, Sergey A. https://orcid.org/0000-0002-0830-7977
Jankásek, Marek https://orcid.org/0000-0002-1467-8790
Reichard, Martin https://orcid.org/0000-0002-9306-0074
Nguyen, Petr https://orcid.org/0000-0003-1395-4287
Sember, Alexandr https://orcid.org/0000-0003-4441-9615
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Funding
Funding for this research was provided by:
Grantová Agentura České Republiky (19-22346Y, 19-22346Y, 19-22346Y, 19-22346Y, 19-22346Y, 19-22346Y, 19-22346Y, 19-22346Y, 19-22346Y)
e-Infrastruktura CZ (e-INFRA LM2018140, e-INFRA LM2018140, e-INFRA LM2018140, e-INFRA LM2018140)
Projects of Large Research, Development and Innovations Infrastructures and the ELIXIR-CZ project (LM2018131, LM2018131, LM2018131, LM2018131)
Spanish Ministry of Universities with European Union’s NextGenerationEU funds (UJAR10MS)
RVO of IAPG CAS (67985904, 67985904, 67985904)
Charles University Research Centre program (204069)
Institute of Animal Physiology and Genetics of the Czech Academy of Sciences
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Article History
Received: 20 May 2023
Revised: 4 October 2023
Accepted: 28 October 2023
First Online: 21 November 2023
Declarations
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: To prevent fish suffering, all handling of fish individuals followed European standards in agreement with §17 of the Act no. 246/1992 Coll. The procedures involving fishes were supervised by the Institutional Animal Care and Use Committee of the Institute of Animal Physiology and Genetics CAS, v.v.i., and the supervisor’s permit no. CZ 02361 was certified and issued by the Ministry of Agriculture of the Czech Republic. The experiments with <i>N. foerschi</i> and <i>N. cardinalis</i> were approved by the Ethics Committee of Severtsov Institute of Ecology and Evolution (Order no. 27 of November 9, 2018). For direct preparations of chromosomes from the kidney, fishes were euthanized using 2-phenoxyethanol (Sigma-Aldrich) before organ sampling. Fin samples (a narrow strip of the caudal fin) were taken from live individuals after fishes were anesthetized using MS-222 (Merck KGaA, Darmstadt, Germany).
: The authors declare no competing interests.
