Samarin, Azin Mohagheghi
Samarin, Azadeh Mohagheghi
Waghmare, Swapnil Gorakh
Danielsen, Marianne
Møller, Hanne Søndergård
Policar, Tomáš
Linhart, Otomar
Dalsgaard, Trine Kastrup
Article History
Received: 28 February 2023
Accepted: 12 November 2023
First Online: 29 November 2023
Declarations
: This study was conducted according to the guidelines of the EU-harmonized Animal Welfare Act of the Czech Republic and approved by the expert committee of the Institutional Animal Care and Use Committee (IACUC) of the University of South Bohemia, Czech Republic. According to the Czech National Directive, the unit is licensed (No. 16OZ19179/2016-17214) (Law against Animal Cruelty, No. 246/1992). The co-authors of this study who were involved in manipulating fish hold certificates giving them the right to work with laboratory animals according to Section 15d, paragraph 3, of Act No. 246/1992 Coll. The study did not involve endangered or protected species. To examine ovulation and to collect gametes, brood fish were anaesthetized with a 0.03 ml/L clove oil water bath to enhance animal welfare and minimize stress. None of the experimental animals, either broodfish or larvae, were sacrificed during this study.
: The authors declare no competing interests.