Kłosiński, Piotr http://orcid.org/0000-0002-6359-9781
Kobak, Jarosław
Augustyniak, Mateusz
Pawlak, Roman
Jermacz, Łukasz
Poznańska-Kakareko, Małgorzata
Kakareko, Tomasz
Funding for this research was provided by:
Narodowe Centrum Nauki (2016/23/B/NZ8/00741)
Article History
Received: 30 January 2021
Revised: 27 October 2021
Accepted: 10 November 2021
First Online: 27 November 2021
Declarations
:
: The authors declare no competing interests.
: We collected and used the fish under permit of the Local Committee for Ethics in Animal Research in Bydgoszcz, Poland, statement no. 50/2017 from 28 September 2017. All procedures using fish met the European Union guidelines on the protection of animals used for scientific purposes (Directive 2010/63/UE). We took care to ensure compliance with animal welfare guidelines to minimize the welfare impact on subjects. We carried out the fishing and transportation with great care to minimise the duration of these operations as we did the direct handling of the fish to avoid any harmful effects (e.g. injuries, scale loss) that might cause undue stress and mortality. During the acclimation period in the stock tanks, we did not observe any negative consequences of transport or stock conditions (the fish were active, foraged and occupied shelters and their mortality was sporadic). During the tests, we noticed neither injuries nor mortality. Each individual was tested only once. After the tests, we released the native gudgeon into the wild, whereas the monkey goby, because of their invasive status, had to be killed (with an overdose of MS-222) and disposed of according to the Regulation of the Polish Minister of the Environment from 9 September 2011 (Journal of Laws No. 210, item 1260). We kept the number of individuals killed in order to obtain the alarm substances to a minimum. Killing was carried out by a qualified person holding an appropriate certificate (No. 2355/2015) issued by the Polish Laboratory Animal Science Association.
: Not applicable.
: Not applicable.