Olivares-Rubio, Hugo F. http://orcid.org/0000-0002-7082-4588
Arce, Elsah http://orcid.org/0000-0002-9815-2525
Burciaga, Luis M. http://orcid.org/0000-0003-4094-3663
Franco, M. http://orcid.org/0000-0003-4954-5100
Osorio-Beristain, Marcela http://orcid.org/0000-0001-7338-1260
Funding for this research was provided by:
Consejo Nacional de Ciencia y Tecnología (Frontier Science Grant (64372))
Animal Behavior Society (Developing Nations Research Grant (108))
Article History
Received: 22 March 2023
Revised: 7 July 2023
Accepted: 8 July 2023
First Online: 21 July 2023
Declarations
:
: The authors have no relevant financial or non-financial interests to disclose.
: All applicable international (The Guide for the Care and Use of Laboratory Animals of the Animal Care Committee of the Animal Behaviour Society) and national (Ley General del Equilibrio Ecológico y la Protección al Ambiente, DOF 13 December 996) guidelines for the care and use of animals were followed. We used the minimum number of fish and the shortest experimental duration necessary to yield clear results and accomplish the experimental goals. After the experiments, fish exposed to Pb were maintained separately and were monitored for 10 days. Fish did not show health problems and did not suffer any apparent injury. No fish died during the experiment and no fish were sacrificed after the experimental procedure. The fish used in this study are not an endangered or protected species.