Fletcher, Paige http://orcid.org/0000-0001-6860-305X
Hamilton, Raymond F. Jr.
Rhoderick, Joseph F.
Postma, Britten
Buford, Mary
Pestka, James J.
Holian, Andrij
Funding for this research was provided by:
National Institute of Environmental Health Sciences (F31 ES028100, R01 ES023209, R01 ES027353)
National Institute of General Medical Sciences (P30 GM103338)
Article History
Received: 1 December 2020
Revised: 30 September 2021
Accepted: 1 October 2021
First Online: 15 October 2021
DECLARATIONS
:
: The content within is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
: The animal use protocol (035-16AHCEHS-062816) was approved by the University of Montana Institutional Animal Care and Use Committee for all mouse studies described within this manuscript. The mice are maintained in microisolation containers within the BSL-2 Laboratory Animal Resources facility at the University of Montana in the accordance with the Guide for the Care and Use of Laboratory Animals. The animal care facility at the University of Montana is staffed with full-time veterinarians that are AAALAC accredited. Mice were monitored on a daily basis along with during/after exposure to particles. Mice were anesthetized with isoflurane before particle or vehicle control exposures so as not to use any restraints or cause distress. All procedures within these studies caused minimal discomfort to the mice; however, in any cases where it was deemed that the mice were in pain or distress (adverse body weight, abnormal activity, poor grooming, abnormal posture), the animal was humanely euthanized.
: Not applicable.
: Not applicable.
: The authors declare no competing interests.