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A simple and cost-effective protocol for high-yield expression of deuterated and selectively isoleucine/leucine/valine methyl protonated proteins in Escherichia coli grown in shaker flasks

Crossref DOI link: https://doi.org/10.1007/s10858-021-00357-x

Published Online: 2021-02-04

Published Print: 2021-03

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Cai, Mengli

Huang, Ying

Lloyd, John

Craigie, Robert

Clore, G. Marius https://orcid.org/0000-0003-3809-1027
Funding

Funding for this research was provided by:

National Institute of Diabetes and Digestive and Kidney Diseases (DK029023)
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Text and Data Mining valid from 2021-02-04

Version of Record valid from 2021-02-04
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Article History

Received: 8 December 2020

Accepted: 20 January 2021

First Online: 4 February 2021

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