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Authors
Ramírez-Molina, Richell Maribet https://orcid.org/0009-0005-3323-6823
García-Ortiz, Humberto https://orcid.org/0000-0002-0453-980X
González-Oliver, Angélica https://orcid.org/0000-0003-4034-8143
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Funding
Funding for this research was provided by:
Dirección General de Asuntos del Personal Académico, UNAM, PAPIIT (IN400423)
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Article History
Received: 18 December 2025
Accepted: 3 February 2026
First Online: 13 February 2026
Declarations
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: The participants have consented to the submission of the article to the journal.
: The authors declare no competing interests.
: Supplementary Table (1) The mean Tm and standard deviation (SD) values obtained by real-time PCR for B and non-B individuals at different DNA concentrations. Supplementary Table (2) The mean Tm values obtained by real-time PCR for the Mazahua samples (DNA concentration to 0.5–1 ng). Supplementary Table (3) ANOVA results for Tm of real-time PCR products. Supplementary Table (4) Tukey test.
: This research was designed in accordance with the Declaration of Helsinki 1964 and was approved by the local the Ethics and Scientific Responsibility Committee of the Facultad de Ciencias, Universidad Nacional Autónoma de México, UNAM, (Approval Number PI_11_01_23).
: Informed consent was obtained from all individual participants included in the study.
