Zhao, Zhanji
Zhu, Jiabing
Zhou, Lijian
Sun, Nan
Chang, Kaile
Hu, Xiaoyue
Hu, Yuting
Ren, Mingzhi
Cheng, Yan
Xu, Derong
Xin, Hongbo
Zhang, Chunbo
Funding for this research was provided by:
National Natural Science Foundation of China (82160259, 31771400, 81860662, 81728006, 81501129)
Natural Science Foundation of Jiangxi Province (20212ACB206039, 20171ACB21001, 20171BCB23029, 20161BAB215200)
Article History
Received: 31 October 2023
Accepted: 20 February 2024
First Online: 9 March 2024
Declarations
:
: All authors have no conflict of interests to disclose.
: All institutional and national guidelines for the care and use of laboratory animals were followed, and they comply with the ARRIVE guidelines. The ethics committee: the Animal Ethics Committee of Nanchang University. The number or ID of the ethics approval: NCULAE-202209280017.Considering the availability of ducks and the advantages of DHBV, we chose ducks as the experimental material. We cumulative used 60 male ducks to study the HGD system and its application. According to the “laboratory animal-Requirements of environment and housing facilities” (GB 14925-2010, Standardization Administration of the People’s Republic of China), all ducks were under the conditions of room temperature (24 ± 0.5 °C), humidity (50 ± 10%), light (12 h light/dark cycle) and were free access to diet. According to the “Technical specifications on euthanasia of laboratory animals” (RB/T 061-2021, Certification and Accreditation Administration of the People’s Republic of China), the ducks were euthanized with excessive isoflurane at the end of study.