Meek, Stephen
Singh-Dolt, Karamjit
Sutherland, Linda
Sharp, Matthew G. F.
Del-Pozo, Jorge
Walker, David
Burdon, Tom
Funding for this research was provided by:
Biotechnology and Biological Sciences Research Council (BB/J004316/1, BB/H012478/1, BB/J004316/1)
Seventh Framework Programme (HEALTH-F4-2010-241504 (EURATRANS))
Article History
Received: 4 April 2024
Accepted: 1 July 2024
First Online: 9 July 2024
Declarations
:
: The authors declare no competing interests.
: This work complied with the Animal Research: Reporting of In vivo Experiments (ARRIVE) guidelines (Percie du Sert et al. ). A total of fourteen rats were used to generate the data for this paper. All animals of the correct genotype were included for analysis. No animals or data were excluded from analysis for any reason. The number of animals used for each experiment and the size of sample sets is noted in the text. Based on previously published work <sup>10</sup> reporting P75NTR KO in mice, it was considered that there might be no overt phenotypic changes in P75NTR KO rats relative to the P75NTR CKO controls. In addition, our P75NTR KO rats were viable and fertile and demonstrated no obvious abnormalities. For these reasons we chose the minimum sample set of nā=ā3 for histological analysis. Animal work conformed to guidelines for animal husbandry according to the UK Home Office and approval by the Roslin Institute Animal Ethics Committee. Animals were naturally mated and sacrificed by a rising concentration of CO<sub>2</sub> followed by cervical dislocation for confirmation of death in accordance with Home Office schedule 1 procedures. Surgical procedures were performed under Procedural Project Licences 6,004,539 and 6,003,887 held by M.G.F.S.