Kurian, Amitha
Elumalai, Preetham http://orcid.org/0000-0002-9689-9502
Article History
Received: 17 April 2020
Accepted: 7 December 2020
First Online: 6 January 2021
Compliance with ethical standards
:
: The authors declare that they have no competing interests
: ProceedingsKUFOS, Panangad, Kochi, Kerala – Committee to review the PhD work proposal for Ms. Amitha – PhD thesis work – constituted – Orders issued.
: No. SOST/PhD001/2019 Date: 30.03.2017 Panagad, Kochi
: OrderedList removed<b>Members</b>OrderedList removedOrderedList removed<b>Serum collection</b> (Doan et al. CitationRef removed)Fish are anaesthetized with clove oil (5 ml L<sup>−1</sup>), followed by blood collection from the caudal vein with a 1 ml plastic syringe sprayed with heparin and stored at 4 °C until the next day. Using a 1-ml syringe, blood is collected from the caudal vein. The collected blood samples are immediately transferred into Eppendorf tubes and are allowed to clot for 1 h at room temperature and 4 h at 4 °C. The samples are centrifuged at 1500×<i>g</i>, for 5 min, at 4 °C in a cooling centrifuge. The serum is finally collected and stored at − 20 °C until assay.
: Not applicable.