Shaapan, Raafat M. http://orcid.org/0000-0002-2620-4189
Abdel-Ghaffar, Fathy A.
Varjabedian, Kohar Garo http://orcid.org/0000-0002-1667-1832
Saad-Alla, Gehad I.
Article History
Received: 21 September 2021
Accepted: 11 October 2021
First Online: 22 October 2021
Declarations
:
: The authors of the current work declare that they have no conflicts of interest in this study.
: The study was ethically cleared and approved by Medical Research Ethical Committee Research, National Statement on Ethical Conduct in Human and animals Research at National Research Centre, Egypt under registration number # 21-054#.
: The freshwater fishes (<i>Clarias gariepinus</i>) are subjected to high infection rate with <i>Cryptosporidium</i> sp.; the drainage canals obtained fishes showed higher <i>Cryptosporidium</i> infection prevalence than that collected from River Nile which indicates an important public health problem and a potential risk of drainage canals in Egypt. ELISA showed higher prevalence of cryptosporidiosis than mZN for the total examined <i>Clarias gariepinus</i> fish. ELISA and PCR will be the "gold standard" where specificity and sensitivity will be very high, ensuring that undiagnosed <i>Cryptosporidium</i> infection does not occur. The phylogenetic analyses in this study confirmed this protozoal organism to be a novel detection of <i>Cryptosporidium molnari</i> in <i>Clarias gariepinus</i> fish.