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Authors
Alhassen, Wedad
Kobayashi, Yuki
Su, Jessica
Robbins, Brianna
Nguyen, Henry
Myint, Thant
Yu, Micah
Nauli, Surya M.
Saito, Yumiko
Alachkar, Amal https://orcid.org/0000-0002-5365-1726
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Funding
Funding for this research was provided by:
University of California, Irvine
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Article History
Received: 30 April 2021
Accepted: 29 July 2021
First Online: 19 October 2021
Declarations
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: All experimental procedures were reviewed and approved by the Hiroshima University Animal Care Committee and met the Japanese Experimental Animal Research Association standards, as defined in the Guidelines for Animal Experiments (1987), and the Institutional Animal Care and Use Committee of the University of California, Irvine. All experiments were performed in compliance with national and institutional guidelines for the care and use of laboratory animals.
: Not applicable.
: Not applicable.
: The authors declare no competing interests.
: AAV5-EF1a-DIO-ChR2-T159c-eYFP # 35509-AAV5: Addgene_35509.AAV8-hSyn-DIO-hM3D(Gq)-mCherry #44361-AAV8:Addgene_44361).Histo VT One: Nacalai Tesque, Kyoto, Japan.Alexa Fluor 488-conjugated donkey anti-rabbit IgG: Thermo Fisher Scientific, Rockford, IL, USA Alexa Fluor 546-conjugated donkey anti-goat IgG: Thermo Fisher Scientific.Anti-MCH antibody: courtesy of W. Vale, Salk Institute, La Jolla, CA, USA.Anti-c-Fos antibody: Invitrogen, USA.Goat anti-human MCHR1: C-17, sc-5534; Santa Cruz Biotechnology, USA.
