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Article History

Received: 29 October 2021

Accepted: 26 March 2022

First Online: 13 April 2022

Change Date: 19 July 2022

Change Type: Update

Change Details: Missing Open Access funding information has been added in the Funding Note.

Declarations

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: All human samples were anonymized. The use of human tissue provided by the MRC Edinburgh Brain Bank was covered by ethical approval from the East of Scotland Research Ethics Service REC 1 (reference number 16/ES/0084). Human brain tissue obtained from the Institute of Neuropathology Brain Bank (HUB-ICO-IDIBELL Biobank, Barcelona, Spain) followed the guidelines of Spanish legislation (Real Decreto 1716/2011) and the approval of the local ethics committee. This study was approved by the institutional review board of Carlo Besta Neurological Institute and performed according to the guidelines approved by the ethics committee. Brain tissue sample from University Hospital of Verona was obtained following the approval of an ethics committee.All procedures involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. Current animal husbandry and housing practices comply with the Council of Europe Convention ETS123 (European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes; Strasbourg, 18.03.1986); Italian Legislative Decree 26/2014, Gazzetta Ufficiale della Repubblica Italiana, 26 July 2014; and with the 86/609/EEC (Council Directive of 24 November 1986 on the approximation of laws, regulations, and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes). The animal facility is licensed and inspected by the Italian Ministry of Health. Mice were housed in groups of 2–5 animals in individually ventilated cages, daily fed, and water provided ad libitum. Lighting was on an automatic 12-h basis. All surgery was performed under tribromoethanol anesthesia, and all efforts were made to minimize suffering and regular veterinary care was daily performed for assessment of animal health. The study, including its Ethics requirements, was approved by the Italian Ministry of Health (Permit Number: NP-02–14).

: Informed consent for participation in research of autopsy tissue was obtained from the relatives of the deceased whenever necessary in accordance with the Declaration of Helsinki (1964–2008) and the Additional Protocol on the Convention of Human Rights and Biomedicine concerning Biomedical Research (2005).

: The authors declare no competing interests.